Imperial College London 2009

Link

http://2009.igem.org/Team:Imperial_College_London/M3

Class

# Format: # Write how you would classify them.

Toxins

Name	Number	Comment
DpnII	BBa_K200009	This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C.
TaqI	BBa_K200010	This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C.

Antagonists

Name	Number	Comment
clts	BBa_K098997	temperature sensitive repressor of cl promoter loses binding efficiency between 35 and 42 degrees celcius
Dam	BBa_K200001	prevents cleavage of DNA by leaky expression of restriction enzymes

Topology

Parts

Name	Number	Comment
temperature-inducible promoter cl	BBa_K098995	This is a heat sensitive cI QPI with a high promoter preceding the repressor
DpnII Composite	BBa_K200009	This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37 deg C.
TaqI Composite	BBa_K200010	This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. The working temperature is 65 deg C.
Dam methylase	BBa_K200001	protects the DNA from cleavage
costitutive promoter	BBa_J23103	constitutive promoter